Rabbit polyclonal antibody to p53 (Phospho-Ser392)
Specificity
The antibody detects endogenous level of p53 only when phosphorylated at serine 392.
Applications
WB
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of serine 392 (P-D-S(p)-D) derived from Human p53.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of extracts from 293 cells treated with Etoposide using Phospho-p53 (Ser392) antibody #11969.The lane on the right is treated with the antigen-specific peptide.
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