Rabbit Polycolonal antibody to Smad1 / 5 / 9 (Phospho-Ser463+Ser465)
Specificity
Smad1 / 5 / 9 (Phospho-Ser463+Ser465) Antibody detects endogenous levels of Smad1 / 5 / 9 only when phosphorylated at Ser463+Ser465
Applications
WB
Reactivity
Human, Rat
Immunogen
A synthesized peptide derived from human Smad1 / 5 / 9 (Phospho-Ser463+Ser465)
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
BSP-1, BSP1, hSMAD1, JV4-1, MAD homolog 1, Mad-related protein 1, MADH1, MADR1, Mothers against decapentaplegic homolog 1, Mothers against DPP homolog 1, SMAD 1, SMAD family member 1, Transforming growth factor-beta-signaling protein 1
Western blot analysis Smad1/5/9 (Phospho-Ser463+Ser465) using EPO treated COS7 whole cell lysates
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