Rabbit polyclonal antibody to Smad2 (Phospho-Ser465)
Specificity
The antibody detects endogenous levels of Smad2 only when phosphorylated at serine 465.
Applications
WB
Dilutions
Western blotting: 1:500~1:1000
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of Serine 465(C-S-S(p)-M-S) derived from Human Smad2.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
hMAD-2, hSMAD2, JV18-1, MAD homolog 2, Mad-related protein 2, MADH2, MADR2, Mothers against decapentaplegic homolog 2, Mothers against DPP homolog 2, SMAD 2, SMAD family member 2
Western blot analysis of extracts from K562 cells using Smad2 (Phospho-Ser465) Antibody #11838.The lane on the right is treated with the antigen-specific peptide.
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