The antibody detects endogenous level of total Smad3 protein.
Applications
WB, IHC, ICC/IF
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around aa.423~427 (C-S-S-V-S) derived from Human Smad3.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
hMAD-3, hSMAD3, JV15-2, MAD homolog 3, Mad3, MADH3, Mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMAD 3, SMAD family member 3
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Smad3 (Ab-425) Antibody #21325 (left) or the same antibody preincubated with blocking peptide (right).
