Rabbit polyclonal antibody to Tau (Phospho-Thr212)
Specificity
The antibody detects endogenous level of Tau only when phosphorylated at threonine 212.
Applications
WB
Dilutions
Western blotting: 1:500~1:1000
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of threonine 212 (S-R-T(p)-P-S) derived from Human Tau.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Predicted MW
48 62 78 kd
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Western blot analysis of extracts from mouse brain tissue using Tau (Phospho-Thr212) Antibody #11257 (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).
Publishing research using Anti-Tau (Phospho-Thr212) Antibody (A51288)? Please let us know so that we can list the citation on this page.