Anti-COL1A2 Antibody (A17214)

Name: Anti-COL1A2 Antibody
Brand: Antibodies.com
SKU: A17214
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to COL1A2 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-COL1A2 Antibody

Description

Rabbit polyclonal antibody to COL1A2.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:25-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 500-600 of human Collagen I/COL1A2 (NP_000080.2).

Sequence

PTGDPGKNGDKGHAGLAGARGAPGPDGNNGAQGPPGPQGVQGGKGEQGPPGPPGFQGLPGPSGPAGEVGKPGERGLHGEFGLPGPAGPRGERGPPGESGAA

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

129 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Alpha 2 collagen type I, Alpha 2 type I collagen, Alpha 2 type I procollagen, Alpha 2(I) collagen, Alpha 2(I) procollagen, Alpha-2 type I collagen, CO1A2_HUMAN, Collagen alpha 2(I) chain, Collagen alpha-2(I) chain, Collagen I alpha 2 polypeptide, Collagen of skin tendon and bone alpha 2 chain, Collagen type I alpha 2, OI4, Osteogenesis imperfecta type IV, Type I procollagen

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-COL1A2 Antibody (A17214)

Western blot analysis of extracts of A375 cells, using Anti-COL1A2 Antibody (A17214) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

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Figure 2: Immunohistochemistry - Anti-COL1A2 Antibody (A17214)

Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-COL1A2 Antibody (A17214) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunofluorescence - Anti-COL1A2 Antibody (A17214)

Immunofluorescence analysis of A-431 cells using Anti-COL1A2 Antibody (A17214) at a dilution of 1:25. DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-COL1A2 Antibody (A17214)

Immunofluorescence analysis of NIH/3T3 cells using Anti-COL1A2 Antibody (A17214) at a dilution of 1:25. DAPI was used to stain the cell nuclei (blue).

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