Anti-Collagen I Antibody (A11439)

Name: Anti-Collagen I Antibody
Brand: Antibodies.com
SKU: A11439
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Collagen I for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-Collagen I Antibody

Description

Rabbit polyclonal antibody to Collagen I.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1100-1200 of human Collagen I/COL1A1 (NP_000079.2).

Sequence

GEQGDRGIKGHRGFSGLQGPPGPPGSPGEQGPSGASGPAGPRGPPGSAGAPGKDGLNGLPGPIGPPGPRGRTGDAGPVGPPGPPGPPGPPGPPSAGFDFSF

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

139 kDa / 220 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Alpha 1 type I collagen, Alpha 2 type I collagen, alpha 2 type I procollagen, alpha 2(I) procollagen, alpha 2(I)-collagen, Alpha-1 type I collagen, alpha1(I) procollagen, CO1A1_HUMAN, COL1A1, COL1A2, collagen alpha 1 chain type I, Collagen alpha-1(I) chain, collagen alpha-1(I) chain preproprotein, Collagen I alpha 1 polypeptide, Collagen I alpha 2 polypeptide, collagen of skin, tendon and bone, alpha-1 chain, collagen of skin, tendon and bone, alpha-2 chain, Collagen type I alpha 1, Collagen type I alpha 2, EDSC, OI1, OI2, OI3, OI4, pro-alpha-1 collagen type 1, type I proalpha 1, Type I procollagen, type I procollagen alpha 1 chain

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (10)

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Figure 1: Western Blot - Anti-Collagen I Antibody (A11439)

Western blot analysis of various lysates, using Anti-Collagen I Antibody (A11439) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Western Blot - Anti-Collagen I Antibody (A11439)

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Figure 3: Immunohistochemistry - Anti-Collagen I Antibody (A11439)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-Collagen I Antibody (A11439) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Collagen I Antibody (A11439)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-Collagen I Antibody (A11439) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.