Anti-Collagen I Antibody (A282758)

Anti-Collagen I Antibody

Rabbit polyclonal antibody to Collagen I.

This antibody recognises both native and heat denatured rat collagen type I.

ELISA, IHC-Fr, IHC-P, IF

ELISA: 1:1500 - 1:6,000, IF: 1:10 - 1:60, IHC-Fr: 1:10 - 1:60, It has been reported that this antibody is suitable for use on paraffin embedded formalin fixed sections following treatment with 1 mg/ml pepsin in 0.5 M acetic acid for 2 hours at 37ºC and multiple buffer washes.

Rat

Type I Collagen from rat tail tendon.

Rabbit

Polyclonal

IgG

Unconjugated

Affinity chromatography on immobilized rat collagen I.

Reconstitution dependent.

Reconstitute with 500 µl of sterile distilled water. Vial contains 0.05 mg of lyophilized antibody.

Supplied in 0.1M Sodium Chloride with 0.01M Sodium Phosphate, 0.01M Sodium Borate, 1% Dextran, and 1% Mannitol.

Lyophilized: Store at 4°C. Reconstituted: Store at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Collagen is located in the extracellular matrix of connective tissues. It is part of the interacting network of proteoglycans and proteins that provides a structural framework for both soft and calcified connective tissues. Type I collagen (~95 kDa) is found in bone, cornea, skin and tendon.Mutations in the encoding gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. The use of Rabbit anti Rat Collagen I antibody in western Blotting is not currently supported, however, it has been reported that this antibody has been used successfully for the quantitation of collagen I in rabbit aortic tissue by western blotting.

Alpha 1 type I collagen, Alpha 2 type I collagen, alpha 2 type I procollagen, alpha 2(I) procollagen, alpha 2(I)-collagen, Alpha-1 type I collagen, alpha1(I) procollagen, CO1A1_HUMAN, COL1A1, COL1A2, collagen alpha 1 chain type I, Collagen alpha-1(I) chain, collagen alpha-1(I) chain preproprotein, Collagen I alpha 1 polypeptide, Collagen I alpha 2 polypeptide, collagen of skin, tendon and bone, alpha-1 chain, collagen of skin, tendon and bone, alpha-2 chain, Collagen type I alpha 1, Collagen type I alpha 2, EDSC, OI1, OI2, OI3, OI4, pro-alpha-1 collagen type 1, type I proalpha 1, Type I procollagen, type I procollagen alpha 1 chain