Anti-Complement factor B Antibody [ARC54100] (A308997)

Name: Anti-Complement factor B Antibody [ARC54100]
Brand: Antibodies.com
SKU: A308997

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This product is discontinued

Anti-Complement factor B Antibody [ARC54100] (A308997) has been discontinued and is no longer available.

View all Anti-Complement factor B Antibodies.

Specifications

Name

Anti-Complement factor B Antibody [ARC54100]

Description

Rabbit monoclonal [ARC54100] antibody to Complement factor B.

Applications

WB, ICC/IF

Dilutions

WB: 1:10,000-1:150,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 400-500 of human Complement factor B (CFB) (NP_001701.2).

Sequence

TVIDEIRDLLYIGKDRKNPREDYLDVYVFGVGPLVNQVNINALASKKDNEQHVFKVKDMENLEDVFYQMIDESQSLSLCGMVWEHRKGTDYHKQPWQAKIS

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC54100

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

100 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

C3/C5 convertase, Glycine-rich beta glycoprotein, PBF2, Properdin factor B

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-Complement factor B Antibody [ARC54100] (A308997)

Western blot analysis of extracts of Human plasma, using Anti-Complement factor B Antibody [ARC54100] (A308997) at 1:130000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 60s.

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Figure 2: Immunofluorescence - Anti-Complement factor B Antibody [ARC54100] (A308997)

Immunofluorescence analysis of RAW 246 7 cells using Anti-Complement factor B Antibody [ARC54100] (A308997) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 3: Immunofluorescence - Anti-Complement factor B Antibody [ARC54100] (A308997)

Immunofluorescence analysis of THP-1 using Anti-Complement factor B Antibody [ARC54100] (A308997) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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