Rabbit polyclonal antibody to Cortactin (Phospho-Tyr466)
Specificity
The antibody detects endogenous level of Cortactin only when phosphorylated at tyrosine 466.
Applications
WB
Dilutions
Western blotting: 1:500~1:1000
Reactivity
Human, Mouse
Immunogen
Peptide sequence around phosphorylation site of tyrosine 466 (P-V-Y(p)-E-T) derived from Human CORTACTIN.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0mg / ml
Predicted MW
85kd
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Western blot analysis of extracts from JK cells, treated with H2O2 or calf intestinal phosphatase (CIP), using Cortactin (Phospho-Tyr466) Antibody #11272.
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