Anti-CTLA4 Antibody [1B8] (A279084)

Anti-CTLA4 Antibody [1B8]

Hamster monoclonal [1B8] antibody to CTLA4.

This antibody recognises mouse CD152, commonly known as CTLA-4 (cytotoxic T-lymphocyte protein 4), a structural homolog for CD28. CD152 binds the same ligands as CD28, namely CD80 (B7.1) and CD86 (B7.2), but with much higher affinity. However, while binding of CD28 enhances T cell function, binding of CD152 inhibits it. CD152 is not detected on naïve T-cells and is induced upon T-cell activation. In mice, lack of CD152 leads to massive lymphoproliferative disorder leading to tissue destruction and autoimmunity, suggesting a critical role for CD152 in limiting T-cell responses. CD152 has been shown to reduce IL-2 production after ligation and can inhibit T-cell expansion. This regulatory role and the high binding affinity of CD152 make it a potential therapy for autoimmune diseases such as rheumatoid arthritis. Hamster anti Mouse CD152 antibody, clone 1B8 may be used to enhance proliferation and pro-inflammatory cytokines of lymph node cells. It may also be used to enhance tumour immunity.

WB, IP, Flow Cytometry, ELISA, IF

Flow Cytometry: 1:10 - 1:100, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl.

Mouse

Extracellular portion of mouse CD152 fused to a mouse IgG2a.

Hamster

Monoclonal

1B8

IgG

Unconjugated

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Hamster anti Mouse CD152 antibody, clone 1B8 recognizes mouse CD152, commonly known as CTLA-4 (cytotoxic T-lymphocyte protein 4), a structural homolog for CD28. CD152 binds the same ligands as CD28, namely CD80 (B7.1) and CD86 (B7.2), but with much higher affinity. However, while binding of CD28 enhances T cell function, binding of CD152 inhibits it. CD152 is not detected on naïve T-cells and is induced upon T-cell activation. In mice, lack of CD152 leads to massive lymphoproliferative disorder leading to tissue destruction and autoimmunity, suggesting a critical role for CD152 in limiting T-cell responses. CD152 has been shown to reduce IL-2 production after ligation and can inhibit T-cell expansion. This regulatory role and the high binding affinity of CD152 make it a potential therapy for autoimmune diseases such as rheumatoid arthritis. Hamster anti Mouse CD152 antibody, clone 1B8 may be used to enhance proliferation and pro-inflammatory cytokines of lymph node cells. It may also be used to enhance tumour immunity.

ALPS5, CD, CD 152, CD152, CD152 antigen, CD152 isoform, Celiac disease 3, CELIAC3, CTLA 4, CTLA-4, CTLA4_HUMAN, Cytotoxic T cell associated 4, Cytotoxic T lymphocyte antigen 4, Cytotoxic T lymphocyte associated 4, Cytotoxic T lymphocyte associated 4, soluble isoform, included, Cytotoxic T lymphocyte associated antigen 4, Cytotoxic T lymphocyte associated antigen 4 short spliced form, Cytotoxic T lymphocyte associated protein 4, Cytotoxic T lymphocyte associated serine esterase 4, Cytotoxic T lymphocyte protein 4, Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4, GRD4, GSE, ICOS, IDDM12, insulin-dependent diabetes mellitus 12, Ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4, OTTHUMP00000216623