Anti-Cyclin B1 Antibody [ARC0474] (A306720)

Name: Anti-Cyclin B1 Antibody [ARC0474]
Brand: Antibodies.com
SKU: A306720
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0474] antibody to Cyclin B1 for WB.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Knockout Validated Antibody

Specifications

Name

Anti-Cyclin B1 Antibody [ARC0474]

Description

Rabbit monoclonal [ARC0474] antibody to Cyclin B1.

Applications

Dilutions

WB: 1:500-1:2,000

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 3-177 of human Cyclin B1 (P14635).

Sequence

LRVTRNSKINAENKAKINMAGAKRVPTAPAATSKPGLRPRTALGDIGNKVSEQLQAKMPMKKEAKPSATGKVIDKKLPKPLEKVPMLVPVPVSEPVPEPEPEPEPEPVKEEKLSPEPILVDTASPSPMETSGCAPAEEDLCQAFSDVILAVNDVDAEDGADPNLCSEYVKDIYAY

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0474

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

55 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CCNB, CCNB 1, ccnb1, CCNB1_HUMAN, G2 mitotic specific cyclin B1, G2/mitotic-specific cyclin-B1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-Cyclin B1 Antibody [ARC0474] (A306720)

Western blot analysis of extracts from wild type (WT) and Cyclin B1 knockout (KO) HeLa cells, using Anti-Cyclin B1 Antibody [ARC0474] (A306720) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Figure 2: Western Blot - Anti-Cyclin B1 Antibody [ARC0474] (A306720)

Western blot analysis of extracts of various cell lines, using Anti-Cyclin B1 Antibody [ARC0474] (A306720) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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