Rabbit polyclonal antibody to Cyclin B1 (phospho-Ser147)
Specificity
The antibody detects endogenous level of Cyclin B1 only when phosphorylated at serine 147.
Applications
WB, IHC, IF
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of Serine 147 (A-F-S(p)-D-V) derived from Human Cyclin B1.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0mg / ml
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Cyclin B1 (Phospho-Ser147) Antibody #11540 (left) or the same antibody preincubated with blocking peptide (right).
![Immunohistochemistry - Anti-Cyclin B1 Antibody [CCNB1/1098] - BSA and Azide free (A253640) - Antibodies.com](https://cdn.antibodies.com/image/catalog/253/A253640_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-Cyclin B1 Antibody [CCNB1/1098] (A250460) - Antibodies.com](https://cdn.antibodies.com/image/catalog/250/A250460_1.jpg?profile=product_alternative)
![SDS-PAGE - Anti-Cyclin B1 Antibody [V92.1] (A250459) - Antibodies.com](https://cdn.antibodies.com/image/catalog/250/A250459_1.jpg?profile=product_alternative)
![SDS-PAGE - Anti-Cyclin B1 Antibody [V92.1] - BSA and Azide free (A253639) - Antibodies.com](https://cdn.antibodies.com/image/catalog/253/A253639_1.jpg?profile=product_alternative)
![Flow Cytometry - Anti-Cyclin B1 Antibody [V152] (A285847) - Antibodies.com](https://cdn.antibodies.com/image/catalog/285/A285848_1.jpg?profile=product_alternative)
![Western Blot - Anti-Cyclin B1 Antibody [ARC2614] (A309196) - Antibodies.com](https://cdn.antibodies.com/image/catalog/309/A309196_1.jpg?profile=product_alternative)