Rabbit polyclonal antibody to Cyclin E1 (phospho Thr395).
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:2,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic phosphorylated peptide around T395 of human Cyclin E1 (NP_001229.1).
Sequence
LLTPP
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Affinity purification.
Molecular Weight
55 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
CCNE, Ccne1, CCNE1_HUMAN, cyclin E variant ex5del, cyclin E variant ex7del, Cyclin Es, Cyclin Et, CyclinE, G1/S specific cyclin E, G1/S-specific cyclin-E1
Figure 1: Western Blot - Anti-Cyclin E1 (phospho Thr395) Antibody (A10865)
Western blot analysis of extracts of various cell lines, using Anti-Cyclin E1 (phospho Thr395) Antibody (A10865). The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA.
Immunofluorescence analysis of C6 cells using Anti-Cyclin E1 (phospho Thr395) Antibody (A10865) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of NIH/3T3 cells using Anti-Cyclin E1 (phospho Thr395) Antibody (A10865) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of U2OS cells using Anti-Cyclin E1 (phospho Thr395) Antibody (A10865) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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