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Anti-Cystatin-B Antibody [ARC2833] (A307531)

Western Blot - Anti-Cystatin-B Antibody [ARC2833] (A307531) - Antibodies.com Western Blot - Anti-Cystatin-B Antibody [ARC2833] (A307531) - Antibodies.com
$470
Product Datasheet

Rabbit monoclonal [ARC2833] antibody to Cystatin-B for WB.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Name
Anti-Cystatin-B Antibody [ARC2833]
Description
Rabbit monoclonal [ARC2833] antibody to Cystatin-B.
Applications
WB
Dilutions
WB: 1:100-1:500
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-98 of human Cystatin B (P04080).
Sequence
MMCGAPSATQPATAETQHIADQVRSQLEEKENKKFPVFKAVSFKSQVVAGTNYFIKVHVGDEDFVHLRVFQSLPHENKPLTLSNYQTNKAKHDELTYF
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC2833
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
13 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
CHROW21, CPI B, CPI-B, CST 6, CST6, CSTB, Cystatin B, Cystatin B (stefin B), CYTB, CYTB_HUMAN, EPM1, EPM1A, Liver thiol proteinase inhibitor, PME, Stefin B, Stefin-B, STF B, STFB, ULD
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Cystatin-B Antibody [ARC2833] (A307531) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Cystatin-B Antibody [ARC2833] (A307531)
Western blot analysis of extracts of various cell lines, using Anti-Cystatin-B Antibody [ARC2833] (A307531) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Western Blot - Anti-Cystatin-B Antibody [ARC2833] (A307531) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-Cystatin-B Antibody [ARC2833] (A307531)
Western blot analysis of extracts of various cell lines, using Anti-Cystatin-B Antibody [ARC2833] (A307531) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
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