Anti-DDDDK Tag Antibody [ARC5111-01] (A305669)

Name: Anti-DDDDK Tag Antibody [ARC5111-01]
Brand: Antibodies.com
SKU: A305669
Price: 335.0 USD
Availability: InStock

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$335

Rabbit monoclonal [ARC5111-01] antibody to DDDDK Tag for WB, ICC/IF, IP and Flow Cytometry.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Tags:

Epitope Tag

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Specifications

Name

Anti-DDDDK Tag Antibody [ARC5111-01]

Description

Rabbit monoclonal [ARC5111-01] antibody to DDDDK Tag.

Applications

WB, ICC/IF, IP, Flow Cytometry

Dilutions

WB: 1:2,000-1:10,000, ICC/IF: 1:50-1:200, IP: 1:100-1:500, Flow Cytometry: 1:50-1:200

Reactivity

Species Independent

Immunogen

A synthetic peptide corresponding to DDDDK tag.

Sequence

DYKDDDDK

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC5111-01

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

56 kDa / 50 kDa / 46 kDa / 68 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

DDDDK epitope tag, DDDK, ddk, DYKDDDDK, DYKDDDDK epitope tag, DYKDDDDK tag, ECS epitope tag, ECS tag, Enterokinase Cleavage Site epitope tag, Enterokinase Cleavage Site tag, FLAG, FLAG tag

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (8)

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Figure 1: Western Blot - Anti-DDDDK Tag Antibody [ARC5111-01] (A305669)

Western blot analysis of 293T, 293T transfected with GSK3B Protein, 293T transfected with BCAT2-N Protein and 293T transfected with BCAT2-C Protein, using Anti-DDDDK Tag Antibody [ARC5111-01] (A305669) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Western Blot - Anti-DDDDK Tag Antibody [ARC5111-01] (A305669)

Western blot analysis of 293F transfected with COPB2-N Protein and 293F transfected with COPB2-C Protein, using Anti-DDDDK Tag Antibody [ARC5111-01] (A305669) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 3: Western Blot - Anti-DDDDK Tag Antibody [ARC5111-01] (A305669)

Western blot analysis of 293T, 293T transfected with IRF1 Protein using Anti-DDDDK Tag Antibody [ARC5111-01] (A305669) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 4: Immunofluorescence - Anti-DDDDK Tag Antibody [ARC5111-01] (A305669)

Immunofluorescence analysis of 293T-Flag-C and 293T-Flag-N and 293T cells using Anti-DDDDK Tag Antibody [ARC5111-01] (A305669) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).