Anti-Dnmt1 Antibody [ARC51345] (A307588)

Name: Anti-Dnmt1 Antibody [ARC51345]
Brand: Antibodies.com
SKU: A307588

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This product is discontinued

Anti-Dnmt1 Antibody [ARC51345] (A307588) has been discontinued and is no longer available.

View all Anti-Dnmt1 Antibodies.

Specifications

Name

Anti-Dnmt1 Antibody [ARC51345]

Description

Rabbit monoclonal [ARC51345] antibody to Dnmt1.

Applications

WB, IHC, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DNMT1 (NP_001370.1).

Sequence

MPARTAPARVPTLAVPAISLPDDVRRRLKDLERDSLTEKECVKEKLNLLHEFLQTEIKNQLCDLETKLRKEELSEEGYLAKVKSLLNKDLSLENGAHAYN

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC51345

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

200 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ADCADN, AIM, CXXC finger protein 9, CXXC-type zinc finger protein 9, CXXC9, DNA (cytosine 5 ) methyltransferase 1, DNA (cytosine-5)-methyltransferase 1, DNA methyltransferase 1, DNA methyltransferase HsaI, DNA methyltransferase M.HsaI., DNA MTase, DNA MTase HsaI, DNMT, DNMT 1, Dnmt1o, DNMT1_HUMAN, FLJ16293, HSN1E, M.HsaI, MCMT, Met1, MGC104992, mMmul, MommeD2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (7)

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Figure 1: Western Blot - Anti-Dnmt1 Antibody [ARC51345] (A307588)

Western blot analysis of various lysates, using Anti-Dnmt1 Antibody [ARC51345] (A307588) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Western Blot - Anti-Dnmt1 Antibody [ARC51345] (A307588)

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Figure 3: Immunohistochemistry - Anti-Dnmt1 Antibody [ARC51345] (A307588)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Dnmt1 Antibody [ARC51345] (A307588) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Dnmt1 Antibody [ARC51345] (A307588)

Immunohistochemistry analysis of paraffin-embedded human tonsil using Anti-Dnmt1 Antibody [ARC51345] (A307588) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.