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Anti-DUSP6 Antibody (A14387)

Western Blot - Anti-DUSP6 Antibody (A14387) - Antibodies.com Immunohistochemistry - Anti-DUSP6 Antibody (A14387) - Antibodies.com Immunofluorescence - Anti-DUSP6 Antibody (A14387) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to DUSP6 for WB, IHC and ICC/IF.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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orders@antibodies.com

Name
Anti-DUSP6 Antibody
Description
Rabbit polyclonal antibody to DUSP6.
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence within amino acids 302-381 of human DUSP6 (NP_001937.2).
Sequence
TVTVAYLMQKLNLSMNDAYDIVKMKKSNISPNFNFMGQLLDFERTLGLSSPCDNRVPAQQLYFTTPSNQNVYQVDSLQST
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
45 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Dual specificity phosphatase 6, Dual specificity phosphatase 6 isoform a, Dual specificity protein phosphatase 6, Dual specificity protein phosphatase PYST1, DUS6_HUMAN, DUSP 6, DUSP 6a, DUSP6a, HH19, MAP kinase phosphatase 3, Mitogen activated protein kinase phosphatase 3, Mitogen-activated protein kinase phosphatase 3, MKP 3, MKP-3, MKP3, PYST 1, PYST1, Serine/threonine specific protein phosphatase
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-DUSP6 Antibody (A14387) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-DUSP6 Antibody (A14387)
Western blot analysis of extracts of various cell lines, using Anti-DUSP6 Antibody (A14387) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry - Anti-DUSP6 Antibody (A14387) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-DUSP6 Antibody (A14387)
Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-DUSP6 Antibody (A14387) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence - Anti-DUSP6 Antibody (A14387) - Antibodies.comEnlarge Image
Figure 3: Immunofluorescence - Anti-DUSP6 Antibody (A14387)
Immunofluorescence analysis of C6 cells using Anti-DUSP6 Antibody (A14387) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Publishing research using Anti-DUSP6 Antibody (A14387)? Please let us know so that we can list the citation on this page.

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