Anti-EEA1 Antibody (A12659)

Name: Anti-EEA1 Antibody
Brand: Antibodies.com
SKU: A12659
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to EEA1 for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Tags:

Early Endosome Marker Endosomal Marker

Specifications

Name

Anti-EEA1 Antibody

Description

Rabbit polyclonal antibody to EEA1.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1182-1411 of human EEA1 (NP_003557.2).

Sequence

LKAAVEQEKRNQQILKDQVKKEEEELKKEFIEKEAKLHSEIKEKEVGMKKHEENEAKLTMQITALNENLGTVKKEWQSSQRRVSELEKQTDDLRGEIAVLEATVQNNQDERRALLERCLKGEGEIEKLQTKVLELQRKLDNTTAAVQELGRENQSLQIKHTQALNRKWAEDNEVQNCMACGKGFSVTVRRHHCRQCGNIFCAECSAKNALTPSSKKPVRVCDACFNDLQG

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

175 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Early endosome antigen 1, Early endosome antigen 1, 162kD, Early endosome associated protein, EEA 1, EEA1_HUMAN, Endosome associated protein p162, Endosome-associated protein p162, MST105, MSTP105, ZFYVE2, Zinc finger FYVE domain containing protein 2, Zinc finger FYVE domain-containing protein 2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-EEA1 Antibody (A12659)

Western blot analysis of extracts of various cell lines, using Anti-EEA1 Antibody (A12659) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Figure 2: Immunohistochemistry - Anti-EEA1 Antibody (A12659)

Immunohistochemistry analysis of paraffin-embedded rat lung using Anti-EEA1 Antibody (A12659) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-EEA1 Antibody (A12659)

Immunohistochemistry analysis of paraffin-embedded human mammary cancer using Anti-EEA1 Antibody (A12659) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-EEA1 Antibody (A12659)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-EEA1 Antibody (A12659) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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