Figure 1: Western Blot - Anti-EF-Ts Antibody [ARC2918] (A307798)
Western blot analysis of extracts of various cell lines, using Anti-EF-Ts Antibody [ARC2918] (A307798) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunofluorescence analysis of HeLa cells using Anti-EF-Ts Antibody [ARC2918] (A307798) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Figure 3: Western Blot - Anti-EF-Ts Antibody [ARC2918] (A307798)
Immunoprecipitation analysis of 300µg extracts of 293T cells using 3µg of Anti-EF-Ts Antibody [ARC2918] (A307798). This Western blot was performed on the immunoprecipitate using Anti-EF-Ts Antibody [ARC2918] (A307798) at a dilution of 1:500.
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