EGFR (phospho Tyr1197) Cell Based ELISA Kit (A103789)

EGFR (phospho Tyr1197) Cell Based ELISA Kit

Cell-based (qualitative)

This EGFR (phospho Tyr1197) Cell Based ELISA Kit allows for the detection of EGFR (phospho Tyr1197) and the effects that certain stimulation conditions have on EGFR (phospho Tyr1197) expression in different cell lines. Qualitative determination of EGFR (phospho Tyr1197) concentration is achieved by an indirect ELISA format. In essence, EGFR (phospho Tyr1197) is captured by Anti-EGFR (phospho Tyr1197) Antibodies while Dye 1-conjugated and Dye 2-conjugated secondary antibodies bind the Fc region of the primary antibody. Through this binding, the dye conjugated to the secondary antibody can emit light at a certain wavelength given proper excitation, hence allowing for a fluorometric detection method. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target RFU values. Anti-EGFR Antibody is also included for normalization purposes. The RFU values obtained for the non-phosphorylated target can be used to normalize the RFU value for the phosphorylated target.

Microplate

> 5000 Cells

Human, Mouse, Rat

Store at + 4°C. Stable for 6 months.

9030024J15Rik, AI552599, Avian erythroblastic leukemia viral (v erb b) oncogene homolog , Avian erythroblastic leukemia viral (verbb) oncogene homolog , Cell growth inhibiting protein 40 , Cell proliferation inducing protein 61 , EGF R, EGFR_HUMAN, Epidermal growth factor receptor, Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) , Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) , erb-b2 receptor tyrosine kinase 1, Erbb, erbb 1, Erbb1, Errp, Erythroblastic leukemia viral (v erb-b) oncogene homolog, HER1, mENA , NISBD2, Oncogen ERBB, Oncogene ERBB , OTTMUSP00000005385, OTTMUSP00000005386, PIG61, Proto oncogene c ErbB 1, Proto-oncogene c-ErbB-1, Receptor tyrosine protein kinase ErbB 1 , Receptor tyrosine protein kinase ErbB1 , Receptor tyrosine-protein kinase ErbB-1, RP23-295E4.1, SA7, Species antigen 7, Urogastrone, v-erb-b Avian erythroblastic leukemia viral oncogen homolog, wa2, Wa5