Anti-ERK2 Antibody (A80506)

Name: Anti-ERK2 Antibody
Brand: Antibodies.com
SKU: A80506
Price: 470.0 USD
Availability: InStock

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$470

Rabbit polyclonal antibody to ERK2 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Knockout Validated Antibody

Specifications

Name

Anti-ERK2 Antibody

Description

Rabbit polyclonal antibody to ERK2.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human ERK2 (NP_620407.1).

Sequence

LNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINLKARNYLLSLPHKNKVPWNRLFPNADSKALDLLDKMLTFNPHK

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

42 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ERK, ERK 2, ERK-2, ERT1, Extracellular Signal Regulated Kinase 2, Extracellular signal-regulated kinase 2, MAP kinase 1, MAP kinase 2, MAP kinase isoform p42, MAPK 1, MAPK 2, Mapk1, MAPK2, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 2, MK01_HUMAN, P38, P40, P41, p42-MAPK, P42MAPK, PRKM1, PRKM2, protein kinase, mitogen-activated, 1, protein kinase, mitogen-activated, 2, protein tyrosine kinase ERK2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-ERK2 Antibody (A80506)

Western blot analysis of extracts of various cell lines, using Anti-ERK2 Antibody (A80506) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

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Figure 2: Western Blot - Anti-ERK2 Antibody (A80506)

Western blot analysis of extracts from normal (control) and ERK2 knockout (KO) HeLa cells, using Anti-ERK2 Antibody (A80506) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

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Figure 3: Immunohistochemistry - Anti-ERK2 Antibody (A80506)

Immunohistochemistry analysis of paraffin-embedded rat spleen using Anti-ERK2 Antibody (A80506) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-ERK2 Antibody (A80506)

Immunohistochemistry analysis of paraffin-embedded human liver using Anti-ERK2 Antibody (A80506) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.