Anti-ERK2 Antibody [ARC51159] (A305893)

Name: Anti-ERK2 Antibody [ARC51159]
Brand: Antibodies.com
SKU: A305893
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC51159] antibody to ERK2 for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-ERK2 Antibody [ARC51159]

Description

Rabbit monoclonal [ARC51159] antibody to ERK2.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human ERK2ERK1/2 (P27361/P28482).

Sequence

FLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINMKARNYLQSLPSKTKVAWAKLFPKSD/LNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINLKARNYLLSLPHKNKVPWNRLFPNADSKALDLLDKMLTFNPHK

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC51159

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

41 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ERK, ERK 2, ERK-2, ERT1, Extracellular Signal Regulated Kinase 2, Extracellular signal-regulated kinase 2, MAP kinase 1, MAP kinase 2, MAP kinase isoform p42, MAPK 1, MAPK 2, Mapk1, MAPK2, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 2, MK01_HUMAN, P38, P40, P41, p42-MAPK, P42MAPK, PRKM1, PRKM2, protein kinase, mitogen-activated, 1, protein kinase, mitogen-activated, 2, protein tyrosine kinase ERK2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-ERK2 Antibody [ARC51159] (A305893)

Western blot analysis of extracts of various cell lines, using Anti-ERK2 Antibody [ARC51159] (A305893) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Immunohistochemistry - Anti-ERK2 Antibody [ARC51159] (A305893)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-ERK2 Antibody [ARC51159] (A305893) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-ERK2 Antibody [ARC51159] (A305893)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-ERK2 Antibody [ARC51159] (A305893) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-ERK2 Antibody [ARC51159] (A305893)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-ERK2 Antibody [ARC51159] (A305893) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.