Anti-FABP7 Antibody [2A84] (A270549)

Immunofluorescent analysis of rat embryonic brain section stained using Anti-FABP7 Antibody [2A84] (A270549), at a dilution of 1:500, in green. The tissue was co-stained using Anti-Vimentin Antibody (A85421), at a dilution of 1:5,000, in red. The Anti-FABP7 Antibody [2A84] (A270549) stains developing astrocytes and radial glia, while the Anti-Vimentin Antibody (A85421) reveals cytoplasmic intermediate filaments. Since the sub cellular distribution of these two proteins is distinct, their expression patterns overlap but are not identical.

Western blot analysis of whole brain and neuron-glial cell culture lysates using Anti-FABP7 Antibody [2A84] (A270549), at a dilution of 1:1,000, in green. The lanes contain samples of: [1] Protein standards, [2] adult rat brain, [3] embryonic (E18) rat brain, and [4] rat neuron-glial cells lysates. The bands at approximately 14 kDa correspond to FABP7, detected only in developing tissue and cells. The same blot was simultaneously probed using Anti-Heat Shock Protein 60 Antibody (A85438), at a dilution of 1:10,000, in red. The bands at 60 kDa bands HSP60 protein, which is labeled in all preparations and acts as a positive loading control.