Anti-FGF1 Antibody [ARC59565] (A309364)

Name: Anti-FGF1 Antibody [ARC59565]
Brand: Antibodies.com
SKU: A309364

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This product is discontinued

Anti-FGF1 Antibody [ARC59565] (A309364) has been discontinued and is no longer available.

View all Anti-FGF1 Antibodies.

Specifications

Name

Anti-FGF1 Antibody [ARC59565]

Description

Rabbit monoclonal [ARC59565] antibody to FGF1.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human FGF1.

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC59565

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

17 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Acidic fibroblast growth factor, aFGF, Beta endothelial cell growth factor, Beta-endothelial cell growth factor, ECGF, ECGF beta, ECGF-beta, ECGFA, ECGFB, Endothelial Cell Growth Factor alpha, Endothelial Cell Growth Factor beta, FGF 1, FGF alpha, FGF1_HUMAN, FGFA, Fibroblast growth factor 1, Fibroblast Growth Factor 1 Acidic, GLIO703, HBGF 1, HBGF-1, HBGF1, Heparin binding growth factor 1, Heparin binding growth factor 1 precursor, Heparin-binding growth factor 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-FGF1 Antibody [ARC59565] (A309364)

Western blot analysis of Mouse brain, using Anti-FGF1 Antibody [ARC59565] (A309364) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Figure 2: Western Blot - Anti-FGF1 Antibody [ARC59565] (A309364)

Western blot analysis of various lysates, using Anti-FGF1 Antibody [ARC59565] (A309364) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 60s.

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Figure 3: Immunofluorescence - Anti-FGF1 Antibody [ARC59565] (A309364)

Immunofluorescence analysis of NIH/3T3 using Anti-FGF1 Antibody [ARC59565] (A309364) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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