Anti-GAD65 Antibody [N-GAD65] (A281319)

Anti-GAD65 Antibody [N-GAD65]

Mouse monoclonal [N-GAD65] antibody to GAD65.

This antibody recognises human glutamate decarboxylase 2, also known as 65 kDa glutamic acid decarboxylase, Glutamate decarboxylase 65 kDa isoform or GAD-65. Glutamate decarboxylase 2 is a 585 amino acid ~65 kDa lipid anchored membrane associated protein responsible for catalysis of ?-aminobutyric acid (GABA) production, the major inhibitory neurotransmitter in the central nervous system and is expressed in the brain and pancreatic beta cells.Glutamate decarboxylase 2 is the 65 kDa isoform of glutamate decarboxylase, encoded by the GAD2 gene and predominantly expressed by nerve termini, as opposed to the 67 kDa isoform (GAD67), predominantly found in the cell body, and encoded by the GAD1 gene. Autoantibodies to Glutamate decarboxylase 2 are detected in autoimmune diseases, including Graves disease , Stiff Man Syndrome and are most prevalent in patients with Type I diabetes mellitus or those at high risk of developing Type I diabetes. The N-Terminal region of GAD65 is essential for targeting the enzyme to GABA-containing secretory vesicles. recognises GAD65 but does not recognize GAD67.

WB, IHC-P, IP, IHC-Fr, RIA

WB: 1:100 - 1:1,000

Human, Rat, Monkey

Synthetic peptide sequence PGSGFWSFGSEDGSGDSEN corresponding to amino acids 4-22 within the N-terminus of human GAD65, conjugated to Keyhole Limpet Haemocyanin.

PGSGFWSFGSEDGSGDSEN

Mouse

Monoclonal

N-GAD65

IgG1

Unconjugated

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human Glutamate Decarboxylase 2 (N-Terminal) antibody, clone N-GAD65 recognizes human glutamate decarboxylase 2, also known as 65 kDa glutamic acid decarboxylase, Glutamate decarboxylase 65 kDa isoform or GAD-65. Glutamate decarboxylase 2 is a 585 amino acid ~65 kDa lipid anchored membrane associated protein responsible for catalysis of ?-aminobutyric acid (GABA) production, the major inhibitory neurotransmitter in the central nervous system (Fenalti et al. 2007) and is expressed in the brain and pancreatic beta cells.Glutamate decarboxylase 2 is the 65 kDa isoform of glutamate decarboxylase, encoded by the GAD2 gene and predominantly expressed by nerve termini, as opposed to the 67 kDa isoform (GAD67), predominantly found in the cell body, and encoded by the GAD1 gene. Autoantibodies to Glutamate decarboxylase 2 are detected in autoimmune diseases, including Graves disease (Kallmann et al. 1997), Stiff Man Syndrome (Raju et al. 2005) and are most prevalent in patients with Type I diabetes mellitus or those at high risk of developing Type I diabetes (Petersen et al. 1994). The N-Terminal region of GAD65 is essential for targeting the enzyme to GABA-containing secretory vesicles (Shi et al. 1994). Mouse anti Human Glutamate Decarboxylase 2 (N-Terminal) antibody, clone N-GAD65 recognizes GAD65 but does not recognize GAD67 (Hampe et al. 2001).

65 kDa glutamic acid decarboxylase, DCE 2, DCE2, DCE2_HUMAN, GAD 2, GAD 65, GAD-2, GAD-65, Gad2, Glutamate decarboxylase 2, Glutamate decarboxylase 2 (pancreas), Glutamate Decarboxylase 2 (pancreatic islets and brain 65kDa), Glutamate Decarboxylase 65, Glutamate decarboxylase 65 kDa isoform, Glutamic Acid Decarboxylase 2, Glutamic Acid Decarboxylase 65, MGC161605, MGC161607