Anti-GATA3 Antibody [1A12-1D9] (A279065)

Anti-GATA3 Antibody [1A12-1D9]

Mouse monoclonal [1A12-1D9] antibody to GATA3.

This antibody recognises GATA3 also known as trans-acting T-cell-specific transcription factor GATA-3 or GATA-binding factor 3.The GATA3 protein is a member of the GATA family of transcription factors which has received its name from binding DNA sequences containing the GATA consensus motif 5'-[AT]GATA[AG]-3' (P15976). GATA3 has been shown to play a critical role in T cell development and the differentiation of T helper 2 (Th2) cells. In addition, GATA3 has been implied to have tissue specific functions such as a regulatory role in the development of the mammary gland epithelium and skin tissue. Aberrant GATA3 levels have also been identified in a variety of cancers including breast cancer. Indeed, GATA3 has become a marker for certain breast cancer types and GATA3 antibodies are commonly used for the immunohistochemical evaluation of breast cancer samples.

WB, IHC-P, IP, IF, ICC

ICC: 1:50 - 1:100, IF: 1:50 - 1:100, IHC-P: 1:200 - 1:2,000, IP: 5 ug, WB: 1:1,000

Human, Mouse, Rat

Purified partial recombinant recombinant human GATA3 protein expressed in E. coli.

Mouse

Monoclonal

1A12-1D9

IgG1

Unconjugated

Protein A affinity chromatography of tissue culture supernatant.

Approximately 55 kDa in SH-SY5Y cell lysate and SH-SY5Y nuclear extract.

Liquid

Supplied in Phosphate Buffered Saline with 1 mg/ml BSA, 30% Glycerol, and <0.1% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti GATA3 antibody clone 1A12-1D9 recognizes GATA3 also known as trans-acting T-cell-specific transcription factor GATA-3 or GATA-binding factor 3.The GATA3 protein is a member of the GATA family of transcription factors which has received its name from binding DNA sequences containing the GATA consensus motif 5'-[AT]GATA[AG]-3' (P15976). GATA3 has been shown to play a critical role in T cell development and the differentiation of T helper 2 (Th2) cells (Ho et al. 1991). In addition, GATA3 has been implied to have tissue specific functions such as a regulatory role in the development of the mammary gland epithelium and skin tissue (Chou et al. 2010).Aberrant GATA3 levels have also been identified in a variety of cancers including breast cancer. Indeed, GATA3 has become a marker for certain breast cancer types and GATA3 antibodies are commonly used for the immunohistochemical evaluation of breast cancer samples (Krings et al. 2014).

GATA 3, GATA binding factor 3, GATA binding protein 3, GATA-binding factor 3, GATA3_HUMAN, HDR, HDRS, MGC2346, MGC5199, MGC5445, Trans acting T cell specific transcription factor GATA 3, Trans-acting T-cell-specific transcription factor GATA-3