Anti-GATA3 Antibody [ARC0115] (A307313)

Name: Anti-GATA3 Antibody [ARC0115]
Brand: Antibodies.com
SKU: A307313
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0115] antibody to GATA3 for WB, IP and ChIP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-GATA3 Antibody [ARC0115]

Description

Rabbit monoclonal [ARC0115] antibody to GATA3.

Applications

WB, IP, ChIP

Dilutions

WB: 1:500-1:2,000, IP: 1:100-1:500, ChIP: 1:100-1:500

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human GATA3 (P23771).

Sequence

MEVTADQPRWVSHHHPAVLNGQHPDTHHPGLSHSYMDAAQYPLPEEVDVLFNIDGQGNHVPPYYGNSVRATVQRYPPTHHGSQVCRPPLLHGSLPWLDGGKALGSHHTASPWNLSPFSKTSIHHGSPGPLSVYPPASSSSLSGGHASPHLFTFPPTPPKDVSPDPSLSTPGSAGSARQDEKECLKYQVPLPDSMKLESSH

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0115

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

48 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

GATA 3, GATA binding factor 3, GATA binding protein 3, GATA-binding factor 3, GATA3_HUMAN, HDR, HDRS, MGC2346, MGC5199, MGC5445, Trans acting T cell specific transcription factor GATA 3, Trans-acting T-cell-specific transcription factor GATA-3

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-GATA3 Antibody [ARC0115] (A307313)

Western blot analysis of extracts of various cell lines, using Anti-GATA3 Antibody [ARC0115] (A307313) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Western Blot - Anti-GATA3 Antibody [ARC0115] (A307313)

Immunoprecipitation analysis of 300µg extracts of Jurkat cells using 3µg of Anti-GATA3 Antibody [ARC0115] (A307313). This Western blot was performed on the immunoprecipitate using Anti-GATA3 Antibody [ARC0115] (A307313) at a dilution of 1:500.

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Figure 3: Chromatin Immunoprecipitation - Anti-GATA3 Antibody [ARC0115] (A307313)

Chromatin immunoprecipitation (ChIP) analysis of extracts of MCF7 cells, using Anti-GATA3 Antibody [ARC0115] (A307313) and Rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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