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Anti-Glypican 3 Antibody [ARC0675] (A308158)

Western Blot - Anti-Glypican 3 Antibody [ARC0675] (A308158) - Antibodies.com Immunofluorescence - Anti-Glypican 3 Antibody [ARC0675] (A308158) - Antibodies.com
$470
Product Datasheet

Rabbit monoclonal [ARC0675] antibody to Glypican 3 for WB and ICC/IF.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-Glypican 3 Antibody [ARC0675]
Description
Rabbit monoclonal [ARC0675] antibody to Glypican 3.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:2,000, ICC/IF: 1:50-1:200
Reactivity
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 21-211 of human Glypican 3 (GPC3) (P51654).
Sequence
PGQAQPPPPPPDATCHQVRSFFQRLQPGLKWVPETPVPGSDLQVCLPKGPTCCSRKMEEKYQLTARLNMEQLLQSASMELKFLIIQNAAVFQEAFEIVVRHAKNYTNAMFKNNYPSLTPQAFEFVGEFFTDVSLYILGSDINVDDMVNELFDSLFPVIYTQLMNPGLPDSALDINECLRGARRDLKVFGNF
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC0675
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
70 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
DGSX, Glypican proteoglycan 3, Glypican-3 [Precursor], Gpc3, GPC3_HUMAN, GTR2 2, GTR2-2, Heparan sulphate proteoglycan, Intestinal protein OCI 5, Intestinal protein OCI-5, MXR7, OCI 5, OCI-5, OCI5, SDYS, Secreted glypican-3, SGB, SGBS, SGBS1
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Glypican 3 Antibody [ARC0675] (A308158) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Glypican 3 Antibody [ARC0675] (A308158)
Western blot analysis of extracts of HepG2 cells, using Anti-Glypican 3 Antibody [ARC0675] (A308158) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunofluorescence - Anti-Glypican 3 Antibody [ARC0675] (A308158) - Antibodies.comEnlarge Image
Figure 2: Immunofluorescence - Anti-Glypican 3 Antibody [ARC0675] (A308158)
Immunofluorescence analysis of HepG2 cells using Anti-Glypican 3 Antibody [ARC0675] (A308158) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Publishing research using Anti-Glypican 3 Antibody [ARC0675] (A308158)? Please let us know so that we can list the citation on this page.

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