The mesoderm plays a key role in giving rise to a wide array of cell types, including bones, muscles, blood cells, and the circulatory system. Mesoderm lineage markers help identify and characterize the development of various cell types originating from the mesodermal germ layer during embryogenesis. Brachyury, also known as T, is a key transcription factor and a well-established marker of mesoderm lineage. It plays a critical role in mesoderm formation, specifically in the development of notochord, the structure that serves as the foundation for the vertebral column. Brachyury marks the earliest embryonic mesodermal progenitors. Mesoderm posterior (Mesp) genes, Mesp1 and Mesp2, are additional transcription factors that are essential for cardiac and somite development. They are critical for specifying paraxial mesoderm, which gives rise to skeletal muscles and vertebrae. The Wnt signaling pathway is involved in multiple aspects of mesoderm development. Activation of specific Wnt ligands and their downstream targets helps regulate the specification and differentiation of mesodermal lineages. Beta-catenin is often used as a marker for mesodermal commitment. Flk1 (VEGFR2 or KDR) is a receptor for vascular endothelial growth factor (VEGF). It is a marker for cardiovascular mesoderm, including the endothelial cells that form blood vessels and the hematopoietic cells that give rise to blood cells. Flk1-expressing cells are found in the lateral plate mesoderm and are essential for vasculogenesis. Another receptor tyrosine kinase, PDGFRα (Platelet-Derived Growth Factor Receptor Alpha) is expressed in mesodermal cells that give rise to various connective tissues, including cartilage, tendons, and ligaments. It is crucial for the development and maintenance of these tissues. Several other transcription factors have key roles in mesoderm-derived tissues. These include: 1) MyoD, a regulator of skeletal muscle development. It is expressed in committed myoblasts and is responsible for initiating the myogenic differentiation program, leading to the formation of skeletal muscle fibres; 2) GATA4 and GATA6, transcription factors that play crucial roles in heart development. They are expressed in the mesoderm-derived cardiac progenitor cells and are involved in cardiac lineage commitment and differentiation; 3) Runx2, a transcription factor that regulates the differentiation of mesenchymal stem cells into osteoblasts. It is therefore essential for the formation of bones and skeletal tissues; 4) TBX6, involved in somite development and segmentation. It plays a role in the establishment of the vertebral column and axial skeleton; 5) HAND2, a transcription factor expressed in the developing heart and limb bud mesoderm. It is essential for proper heart chamber formation and limb patterning; 6) Scl/TAL1 (Stem Cell leukaemia/T-Cell Acute Lymphocytic leukaemia 1), a transcription factor involved in haematopoiesis. It plays a critical role in the development of blood cells from mesodermal precursors, including erythrocytes and megakaryocytes; 7) NKX2.5, another important transcription factor in cardiac development. It is expressed in cardiac progenitor cells and is necessary for proper heart tube formation and cardiac lineage commitment; 8) Wt1 (Wilms' Tumour 1), a transcription factor involved in the development of the urogenital system. It is expressed in the intermediate mesoderm and plays a role in kidney and gonad development. We offer a large product catalogue of research tools for investigating mesoderm lineage markers, including CD34 antibodies, Glypican 3 antibodies, alpha smooth muscle Actin antibodies, Cardiac Troponin I ELISA Kits, and Glypican 3 ELISA Kits. Explore our full mesoderm lineage markers product range below and discover more, for less.