Anti-GM130 Antibody (A14686)

Name: Anti-GM130 Antibody
Brand: Antibodies.com
SKU: A14686
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to GM130 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Tags:

Golgi Marker

Specifications

Name

Anti-GM130 Antibody

Description

Rabbit polyclonal antibody to GM130.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:1,000-1:5,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-300 of human GM130 (NP_004477.3).

Sequence

SKLAAAKKKLREYQQRNSPGVPTGAKKKKKIKNGSNPETTTSGGCHSPEDTPKDNAATLQPSDDTVLPGGVPSPGASLTSMAASQNHDADNVPNLMDETKTFSSTESLRQLSQQLNGLVCESATCVNGEGPASSANLKDLESRYQQLAVALDSSYVTNKQLNITIEKLKQQNQEITDQLEEEKKECHQKQGALREQLQVHIQTIGILVSEKAELQTALAHTQHAARQKEGESEDLASRLQYSRRRVGELERALSAVSTQQKKADRYNKELTKERDALRLEL

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

130 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

130 kDa cis Golgi matrix protein, 130 kDa cis-Golgi matrix protein, Cis golgi matrix protein GM130, Gm130 autoantigen, GOGA2_HUMAN, GOLGA 2, Golga2, Golgi autoantigen, Golgi autoantigen golgin subfamily a 2, Golgi matrix protein GM130, Golgin 95, golgin A2, Golgin subfamily a 2, Golgin subfamily A member 2, Golgin-95, MGC20672, SY11 protein

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (7)

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Figure 1: Western Blot - Anti-GM130 Antibody (A14686)

Western blot analysis of Mouse heart, using Anti-GM130 Antibody (A14686) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 2: Immunohistochemistry - Anti-GM130 Antibody (A14686)

Immunohistochemistry analysis of paraffin-embedded rat liver using Anti-GM130 Antibody (A14686) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-GM130 Antibody (A14686)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-GM130 Antibody (A14686) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-GM130 Antibody (A14686)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-GM130 Antibody (A14686) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.