Anti-GPC3 Antibody (A90979)

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This product is discontinued

Anti-GPC3 Antibody (A90979) has been discontinued and is no longer available.

View all GPC3 Antibodies.

Specifications

Name

Anti-GPC3 Antibody

Description

Rabbit polyclonal antibody to GPC3.

Applications

WB, IF

Dilutions

WB: 1:500-1:2000, IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 290-550 of human GPC3 (NP_004475.1).

Sequence

VEIDKYWREYILSLEELVNGMYRIYDMENVLLGLFSTIHDSIQYVQKNAGKLTTTIGKLCAHSQQRQYRSAYYPEDLFIDKKVLKVAHVEHEETLSSRRRELIQKLKSFISFYSALPGYICSHSPVAENDTLCWNGQELVERYSQKAARNGMKNQFNLHELKMKGPEPVVSQIIDKLKHINQLLRTMSMPKGRVLDKNLDEEGFESGDCGDDEDECIGGSGDGMIKVKNQLRFLAELAYDLDVDDAPGNSQQATPKDNEIS

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

70kDa

Formulation

Supplied in Phosphate Buffered Saline, pH 7.30, with 0.02% Sodium Azide and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Glypican-3, GTR2-2, Intestinal protein OCI-5, MXR7, OCI5

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-GPC3 Antibody (A90979)

Western blot analysis of extracts of various cell lines, using Anti-GPC3 Antibody (A12384) at 1:1,000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.

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Immunofluorescence - Anti-GPC3 Antibody (A90979)

Immunofluorescence analysis of NIH/3T3 cells using Anti-GPC3 Antibody (A12384) at dilution of 1:100. Blue: DAPI for nuclear staining.

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