Anti-GRP78 Antibody (A305100)

Name: Anti-GRP78 Antibody
Brand: Antibodies.com
SKU: A305100
Price: 520.0 USD
Availability: InStock

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$520

Rabbit polyclonal antibody to GRP78 for WB, IHC, ICC/IF and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Ribosomal Marker

Specifications

Name

Anti-GRP78 Antibody

Description

Rabbit polyclonal antibody to GRP78.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:1,000, ICC/IF: 1:100

Reactivity

Human, Mouse, Rat, Bovine, Fungi, Hamster, Monkey, Rabbit, Xenopus, Canine

Immunogen

Rat GRP78 (Bip) synthetic peptide conjugated to KLH.

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Peptide affinity purification.

Concentration

1 mg/ml

Molecular Weight

~78 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

78 kDa glucose regulated protein, 78 kDa glucose-regulated protein, AL022860, AU019543, BIP, D2Wsu141e, D2Wsu17e, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, Endoplasmic reticulum lumenal Ca2+ binding protein grp78, Epididymis secretory sperm binding protein Li 89n, FLJ26106, Glucose Regulated Protein 78kDa, GRP 78, GRP-78, GRP78 BiP, GRP78_HUMAN, Heat shock 70 kDa protein 5, Heat Shock 70kDa Protein 5, Heat shock protein family A (Hsp70) member 5, HEL S 89n, Hsce70, HSPA 5, HSPA5, Immunoglobulin Heavy Chain Binding Protein, Immunoglobulin heavy chain-binding protein, mBiP, MIF2, Sez7

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-GRP78 Antibody (A305100)

Western blot analysis of rat tissue lysates showing detection of GRP78 protein using Anti-GRP78 Antibody (A305100) at 1:1,000 for 2 hours at room temperature. Load: 15 µgprotein. Block: 1.5% BSA. The secondary antibody used was Donkey Anti-Rabbit IgG: HRP for 1 hour at room temperature.

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Figure 2: Immunofluorescence - Anti-GRP78 Antibody (A305100)

Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-GRP78 Antibody (A305100), at 1:100 for 12 hours at 4°C. The secondary antibody used was APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Magnification: 20x.(A) DAPI (blue) nuclear stain. (B) Anti-GRP78 Antibody. (C) Composite. Heat Shocked at 42°C for 30 min.

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Figure 3: Immunofluorescence - Anti-GRP78 Antibody (A305100)

Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-GRP78 Antibody (A305100), at 1:100 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Magnification: 100x.(A) DAPI (blue) nuclear stain. (B) Anti-GRP78 Antibody. (C) Composite. Heat Shocked at 42°C for 30 min.