Anti-HDLBP Antibody [ARC2855] (A307867)

Name: Anti-HDLBP Antibody [ARC2855]
Brand: Antibodies.com
SKU: A307867

Enlarge Image

This product is discontinued

Anti-HDLBP Antibody [ARC2855] (A307867) has been discontinued and is no longer available.

View all Anti-HDLBP Antibodies.

Specifications

Name

Anti-HDLBP Antibody [ARC2855]

Description

Rabbit monoclonal [ARC2855] antibody to HDLBP.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HDLBP (Q00341).

Sequence

MSSVAVLTQESFAEHRSGLVPQQIKVATLNSEEESDPPTYKDAFPPLPEKAACLESAQEPSGAWGNKIRPIKASVITQVFHVPLEERKYKDMNQFGEGEQ

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2855

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

150 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

HBP, HDL binding protein, HDL-binding protein, high density lipoprotein binding protein, high density lipoprotein binding protein (vigilin), High density lipoprotein-binding protein, PRO2900, VGL, Vigilin, VIGLN_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

Enlarge Image

Figure 1: Western Blot - Anti-HDLBP Antibody [ARC2855] (A307867)

Western blot analysis of extracts of various cell lines, using Anti-HDLBP Antibody [ARC2855] (A307867) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

Enlarge Image

Figure 2: Western Blot - Anti-HDLBP Antibody [ARC2855] (A307867)

Western blot analysis of extracts of Rat pancreas, using Anti-HDLBP Antibody [ARC2855] (A307867) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

Enlarge Image

Figure 3: Immunohistochemistry - Anti-HDLBP Antibody [ARC2855] (A307867)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-HDLBP Antibody [ARC2855] (A307867) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Enlarge Image

Figure 4: Immunohistochemistry - Anti-HDLBP Antibody [ARC2855] (A307867)

Immunohistochemistry analysis of paraffin-embedded mouse pancreas using Anti-HDLBP Antibody [ARC2855] (A307867) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Publishing research using Anti-HDLBP Antibody [ARC2855] (A307867)? Please let us know so that we can list the citation on this page.