Anti-Heme Oxygenase 1 Antibody [1F12-A6] (A304771)

Name: Anti-Heme Oxygenase 1 Antibody [1F12-A6]
Brand: Antibodies.com
SKU: A304771
Price: 505.0 USD
Availability: InStock

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$505

Mouse monoclonal [1F12-A6] antibody to Heme Oxygenase 1 for WB, IHC, ICC/IF, IP and ELISA.

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Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-Heme Oxygenase 1 Antibody [1F12-A6]

Description

Mouse monoclonal [1F12-A6] antibody to Heme Oxygenase 1.

Applications

WB, IHC, ICC/IF, IP, ELISA

Dilutions

WB: 1:1,000, IHC: 1:100, ICC/IF: 1:100

Reactivity

Human, Mouse, Rat, Bovine, Canine, Guinea Pig, Hamster, Monkey, Porcine, Rabbit

Cross Reactivity

This antibody does not cross-react with HO-2.

Immunogen

Human HO-1 synthetic peptide, amino acids 1-30.

Host

Mouse

Clonality

Monoclonal

Clone ID

1F12-A6

Isotype

IgG1

Light Chains

kappa

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

32 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

32 kD, bK286B10, D8Wsu38e, Heat shock protein, heat shock protein 32 kD, heat shock protein 32kD, Heme oxygenase (decycling) 1, Hemox, Hmox, HMOX 1, Hmox1, HMOX1_HUMAN, HO, HO 1, HO-1, HO1, Hsp32

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Immunofluorescence - Anti-Heme Oxygenase 1 Antibody [1F12-A6] (A304771)

Immunocytochemistry/Immunofluorescence analysis of human HaCaT cells, fixed in cold 100% methanol for 10 minutes at -20°C, using Anti-Heme Oxygenase 1 Antibody [1F12-A6] (A304771), at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature. Localization: Cell-cell border staining in epidermis, punctuate nuclear staining..

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Figure 2: Western Blot - Anti-Heme Oxygenase 1 Antibody [1F12-A6] (A304771)

Western blot analysis of human Cervical cancer cell line (HeLa) lysate showing detection of HO-1 protein using Anti-Heme Oxygenase 1 Antibody [1F12-A6] (A304771) at 1:1,000 for 2 hours at room temperature. Load: 15 µg. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Sheep Anti-Mouse IgG: HRP for 1 hour at room temperature.

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Figure 3: Immunofluorescence - Anti-Heme Oxygenase 1 Antibody [1F12-A6] (A304771)

Immunocytochemistry/Immunofluorescence analysis of human cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-Heme Oxygenase 1 Antibody [1F12-A6] (A304771), at 1:100 for 12 hours at 4°C. The secondary antibody used was R-PE Goat Anti-Mouse (yellow) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Microsome. Endoplasmic reticulum. Localizes to the nucleus upon hypoxia. Magnification: 20x.(A) DAPI (blue) nuclear stain. (B) Anti-HO-1 Antibody. (C) Composite.

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Figure 4: Immunohistochemistry - Anti-Heme Oxygenase 1 Antibody [1F12-A6] (A304771)

Immunohistochemistry analysis of mouse backskin, fixed in Bouin's fixative solution and paraffin-embedded. The Primary Antibody used was Anti-Heme Oxygenase 1 Antibody [1F12-A6] (A304771) at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature. Localization: muscle, dermis, hair follicles, epidermis: nuclear everywhere and some cytoplasmic staining.