Anti-Heme Oxygenase 1 Antibody [6B8-2F2] (A304770)

Name: Anti-Heme Oxygenase 1 Antibody [6B8-2F2]
Brand: Antibodies.com
SKU: A304770
Price: 500.0 USD
Availability: InStock

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$500

Mouse monoclonal [6B8-2F2] antibody to Heme Oxygenase 1 for WB and ICC/IF.

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Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-Heme Oxygenase 1 Antibody [6B8-2F2]

Description

Mouse monoclonal [6B8-2F2] antibody to Heme Oxygenase 1.

Applications

WB, ICC/IF

Dilutions

WB: 1:1,000, ICC/IF: 1:100

Reactivity

Human, Mouse, Rat

Cross Reactivity

This antibody does not cross-react with HO-2.

Immunogen

His-tagged Rat HO-1.

Host

Mouse

Clonality

Monoclonal

Clone ID

6B8-2F2

Isotype

IgG1

Light Chains

kappa

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~32 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 50% Glycerol and 0.1% Sodium Azide

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

32 kD, bK286B10, D8Wsu38e, Heat shock protein, heat shock protein 32 kD, heat shock protein 32kD, Heme oxygenase (decycling) 1, Hemox, Hmox, HMOX 1, Hmox1, HMOX1_HUMAN, HO, HO 1, HO-1, HO1, Hsp32

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-Heme Oxygenase 1 Antibody [6B8-2F2] (A304770)

Western blot analysis of Human, mouse, rat Rat kidney Lysate showing detection of ~31 kDa HO-1 protein using Anti-Heme Oxygenase 1 Antibody [6B8-2F2] (A304770) at 1:1,000 for 1 hour at room temperature. Lane 1: MW Ladder. Lane 2: rat kidney Lysate. Block: 5% milk + TBST for 1 hour at room temperature. The secondary antibody used was HRP Goat Anti-Mouse at 1:50 for 1 hour at room temperature. Color Development: TMB solution for 5 minutes at room temperature. Predicted/Observed Size: ~31 kDa.

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Figure 2: Immunofluorescence - Anti-Heme Oxygenase 1 Antibody [6B8-2F2] (A304770)

Immunocytochemistry/Immunofluorescence analysis of mouse fibroblast cell line (NIH 3T3), fixed in 4% formaldehyde for 15 min at room temperature, using Anti-Heme Oxygenase 1 Antibody [6B8-2F2] (A304770), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:5,000 for 5 minutes room temperature. Localization: Nucleus, Cytoplasm. Magnification: 60X.

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