Recombinant Anti-Histone H2A.Z (acetyl Lys4) Antibody [RM221] (A121242)

Name: Anti-Histone H2A.Z (acetyl Lys4) Antibody [RM221]
Brand: Antibodies.com
SKU: A121242
Price: 565.0 USD
Availability: InStock

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$565

Recombinant rabbit monoclonal [RM221] antibody to Histone H2A.Z (acetyl Lys4) for WB, ELISA, Multiplex Protein Detection and ICC.

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Freight Charges

$40 to United States

Lead Time

4-6 Business Days

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Recombinant Antibody Nuclear Marker

Specifications

Name

Anti-Histone H2A.Z (acetyl Lys4) Antibody [RM221]

Description

Recombinant rabbit monoclonal [RM221] antibody to Histone H2A.Z (acetyl Lys4).

Specificity

This antibody recognizes Histone H2A.Z acetylated at Lysine 4.

Applications

WB, ELISA, Multiplex Protein Detection, ICC

Dilutions

WB: 0.5-2 µg/ml, ELISA: 0.2-1 µg/ml, Multiplex Protein Detection: 0.05-0.5 µg/ml

Reactivity

All Vertebrates

Cross Reactivity

There is no cross reactivity with non-modified Lysine 4 or other acetylated Lysines in Histone H2A.

Immunogen

Synthetic peptide corresponding to Histone H2A.Z (acetyl Lys4).

Host

Rabbit

Clonality

Monoclonal

Clone ID

RM221

Isotype

IgG

Conjugate

Unconjugated

Purification

Protein A chromatography.

Concentration

1 mg/ml

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 50% Glycerol, 1% BSA, and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

H2A histone family member Z, H2A.z, H2A/z, H2afz, H2AZ, H2AZ_HUMAN, MGC117173

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Anti-Histone H2A.Z (acetyl Lys4) Antibody [RM221] (A121242)

Anti-Histone H2A.Z (acetyl Lys4) Antibody [RM221] specifically reacts to Histone H2A.Z acetylated at Lysine 4 (K4ac). There is no cross reactivity with non-modified Lysine 4 or other acetylated Lysines in Histone H2A.

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Figure 2: Western Blot - Anti-Histone H2A.Z (acetyl Lys4) Antibody [RM221] (A121242)

Western blot of acid extracts from HeLa cells treated (+) or untreated (-) with sodium butyrate, using Anti-Histone H2A.Z (acetyl Lys4) Antibody [RM221] at 0.5µg/ml, showed a band of Histone H2A.Z acetylated Lysine 4 in treated HeLa cells.

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Figure 3: Immunocytochemistry - Anti-Histone H2A.Z (acetyl Lys4) Antibody [RM221] (A121242)

Immunocytochemical staining of HeLa cells treated with sodium butyrate, using Anti-Histone H2A.Z (acetyl Lys4) Antibody [RM221] (red). Actin filaments have been labeled with fluorescein phalloidin (green).

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