Anti-Histone H3 (phospho S28) Antibody (A16443)

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This product is discontinued

Anti-Histone H3 (phospho S28) Antibody (A16443) has been discontinued and is no longer available.

View all Histone H3 Antibodies.

Specifications

Name

Anti-Histone H3 (phospho S28) Antibody

Description

Rabbit polyclonal antibody to Histone H3 (phospho S28).

Applications

WB, IF

Dilutions

WB: 1:500-1:2000, IF: 1:20-1:100

Reactivity

Human, Rat

Immunogen

A phospho specific peptide corresponding to residues surrounding S28 of human HIST1H3B.

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

17kDa

Formulation

Supplied in Phosphate Buffered Saline, pH 7.30, with 0.02% Sodium Azide and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

H3-3A, H3-5, H3-clustered histone 13, H3-clustered histone 14, H3-clustered histone 15, H3.3A, H3C1, H3C15, H3F3, H3F3A, H3F3C, H3FA, HIST1H3A, HIST2H3A, Histone H3.1, Histone H3.2, Histone H3.3, Histone H3.3C, Histone H3.5, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/m, Histone H3/o

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-Histone H3 (phospho S28) Antibody (A16443)

Western blot analysis of extracts of various cell lines, using Anti-Histone H3 (phospho S28) Antibody (AP0097) at 1:1,000 dilution. HeLa cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight. C6 cells were treated by UV for 15-30 minutes. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

Enlarge Image

Immunofluorescence - Anti-Histone H3 (phospho S28) Antibody (A16443)

Immunofluorescence analysis of U2OS cells using Anti-Histone H3 (phospho S28) Antibody (AP0097). Blue: DAPI for nuclear staining.

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Anti-Histone H3 (phospho S28) Antibody (A16443)

Specifications

Scientific Validation DataValidation Data (2)

Western Blot - Anti-Histone H3 (phospho S28) Antibody (A16443)

Immunofluorescence - Anti-Histone H3 (phospho S28) Antibody (A16443)

Most popular Anti-Histone H3 Antibodies

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