Anti-HSD17B1 Antibody (A16800)

Name: Anti-HSD17B1 Antibody
Brand: Antibodies.com
SKU: A16800
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to HSD17B1 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

orders@antibodies.com

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Specifications

Name

Anti-HSD17B1 Antibody

Description

Rabbit polyclonal antibody to HSD17B1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-328 of human HSD17B1 (NP_000404.2).

Sequence

MARTVVLITGCSSGIGLHLAVRLASDPSQSFKVYATLRDLKTQGRLWEAARALACPPGSLETLQLDVRDSKSVAAARERVTEGRVDVLVCNAGLGLLGPLEALGEDAVASVLDVNVVGTVRMLQAFLPDMKRRGSGRVLVTGSVGGLMGLPFNDVYCASKFALEGLCESLAVLLLPFGVHLSLIECGPVHTAFMEKVLGSPEEVLDRTDIHTFHRFYQYLAHSKQVFREAAQNPEEVAEVFLTALRAPKPTLRYFTTERFLPLLRMRLDDPSGSNYVTAMHREVFGDVPAKAEAGAEAGGGAGPGAEDEAGRGAVGDPELGDPPAAPQ

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

35 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

17 beta HSD 1, 17 beta hydroxysteroid dehydrogenase type 1, 17-beta-HSD 1, 17-beta-hydroxysteroid dehydrogenase type 1, 20 alpha-hydroxysteroid dehydrogenase, 20-alpha-HSD, DHB1_HUMAN, E17KSR, E2DH, EDH17B1, EDH17B2, EDHB17, Estradiol 17 beta dehydrogenase 1, Estradiol 17-beta-dehydrogenase 1, HSD17, Hydroxysteroid (17 beta) dehydrogenase 1, MGC13814, Placental 17 beta hydroxysteroid dehydrogenase, Placental 17-beta-hydroxysteroid dehydrogenase, SDR28C1, Short chain dehydrogenase/reductase family 28CE,member 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-HSD17B1 Antibody (A16800)

Western blot analysis of extracts of various cell lines, using Anti-HSD17B1 Antibody (A16800) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Figure 2: Immunohistochemistry - Anti-HSD17B1 Antibody (A16800)

Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-HSD17B1 Antibody (A16800) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-HSD17B1 Antibody (A16800)

Immunohistochemistry analysis of paraffin-embedded mouse heart using Anti-HSD17B1 Antibody (A16800) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-HSD17B1 Antibody (A16800)

Immunofluorescence analysis of C6 cells using Anti-HSD17B1 Antibody (A16800) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).