Anti-Hsp90 alpha Antibody (A87729)

Name: Anti-Hsp90 alpha Antibody
Brand: Antibodies.com
SKU: A87729

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This product is discontinued

Anti-Hsp90 alpha Antibody (A87729) has been discontinued and is no longer available.

View all Anti-Hsp90 alpha Antibodies.

Specifications

Name

Anti-Hsp90 alpha Antibody

Description

Rabbit polyclonal antibody to Hsp90 alpha.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 629-732 of human Hsp90a (NP_005339.3).

Sequence

AAKKHLEINPDHSIIETLRQKAEADKNDKSVKDLVILLYETALLSSGFSLEDPQTHANRIYRMIKLGLGIDEDDPTADDTSAAVTEEMPPLEGDDDTSRMEEVD

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

101 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

EL52, epididymis luminal secretory protein 52, Heat shock 86 kDa, heat shock 90kD protein 1, alpha, Heat shock 90kD protein 1, alpha like 4, heat shock 90kD protein, alpha-like 4, Heat shock 90kDa protein 1 alpha, Heat shock protein 90kDa alpha (cytosolic) class A member 1, Heat shock protein HSP 90-alpha, HS90A_HUMAN, HSP 86, HSP86, Hsp89, HSP89A, Hsp90, HSP90A, HSP90AA1, HSP90ALPHA, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, LAP 2, LAP2, lipopolysaccharide-associated protein 2, LPS-associated protein 2, Renal carcinoma antigen NY-REN-38

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-Hsp90 alpha Antibody (A87729)

Western blot analysis of extracts of various cell lines, using Anti-Hsp90 alpha Antibody (A87729) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Immunohistochemistry - Anti-Hsp90 alpha Antibody (A87729)

Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-Hsp90 alpha Antibody (A87729) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Hsp90 alpha Antibody (A87729)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-Hsp90 alpha Antibody (A87729) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Hsp90 alpha Antibody (A87729)

Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-Hsp90 alpha Antibody (A87729) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.