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Anti-HSP90 beta Antibody (A304780)

Western Blot - Anti-HSP90 beta Antibody (A304780) - Antibodies.com Immunocytochemistry/Immunofluorescence - Anti-HSP90 beta Antibody (A304780) - Antibodies.com Immunohistochemistry - Anti-HSP90 beta Antibody (A304780) - Antibodies.com Western Blot - Anti-HSP90 beta Antibody (A304780) - Antibodies.com Immunocytochemistry/Immunofluorescence - Anti-HSP90 beta Antibody (A304780) - Antibodies.com
$425
Product Datasheet

Rabbit polyclonal antibody to HSP90 beta for WB, IHC, ICC/IF and ELISA.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Name
Anti-HSP90 beta Antibody
Description
Rabbit polyclonal antibody to HSP90 beta.
Applications
WB, IHC, ICC/IF, ELISA
Dilutions
WB: 1:1,000, IHC: 1:100, ICC/IF: 1:120
Reactivity
Human, Mouse, Rat
Cross Reactivity
This antibody does not cross-react with HSP90 alpha.
Immunogen
Full length Human Hsp90 protein.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Peptide affinity purification.
Concentration
1 mg/ml
Molecular Weight
~90 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
90 kda heat shock protein beta HSP90 beta, D6S182, FLJ26984, Heat shock 84 kDa, Heat shock 90kD protein 1, beta, Heat shock 90kDa protein 1 beta, Heat shock protein 90 alpha family class B member 1, Heat shock protein 90 kDa, Heat shock protein 90kDa alpha (cytosolic) class B member 1, Heat shock protein 90kDa alpha family class B member 1, Heat shock protein beta, Heat shock protein HSP 90 beta, Heat shock protein HSP 90-beta, HS90B_HUMAN, HSP 84, HSP 90, HSP 90 b, HSP 90b, HSP84, hsp90ab1, HSP90B, HSPC2, HSPCB
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-HSP90 beta Antibody (A304780) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-HSP90 beta Antibody (A304780)
Western blot analysis of human Cell line lysates showing detection of HSP90 beta protein using Anti-HSP90 beta Antibody (A304780) at 1:2000 for 2 hours at room temperature. Load: 15 µgprotein. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Donkey Anti-Rabbit IgG: HRP for 1 hour at room temperature.
Immunocytochemistry/Immunofluorescence - Anti-HSP90 beta Antibody (A304780) - Antibodies.comEnlarge Image
Figure 2: Immunofluorescence - Anti-HSP90 beta Antibody (A304780)
Immunocytochemistry/Immunofluorescence analysis of human cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-HSP90 beta Antibody (A304780), at 1:120 for 12 hours at 4°C. The secondary antibody used was R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Cytoplasm. Melanosome. Magnification: 20x.(A) DAPI (blue) nuclear stain. (B) Anti-Hsp90 beta Antibody. (C) Composite.
Immunohistochemistry - Anti-HSP90 beta Antibody (A304780) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-HSP90 beta Antibody (A304780)
Immunohistochemistry analysis of mouse backskin, fixed in Bouin's fixative solution. The Primary Antibody used was Anti-HSP90 beta Antibody (A304780) at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at room temperature. Localization: Cytoplasm.
Western Blot - Anti-HSP90 beta Antibody (A304780) - Antibodies.comEnlarge Image
Figure 4: Western Blot - Anti-HSP90 beta Antibody (A304780)
Western blot analysis of human Cell line lysates showing detection of HSP90 beta protein using Anti-HSP90 beta Antibody (A304780) at 1:1,000 for 2 hours at room temperature. Load: 15 µgprotein. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Donkey Anti-Rabbit IgG: HRP for 1 hour at room temperature. Left: 2 ug of Hsp90 Alpha, Right: 2 ug Hsp90 Beta.
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