Anti-HSP90 beta Antibody [H9010] (A305130)

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$520

Mouse monoclonal [H9010] antibody to HSP90 beta for WB, IHC, ICC/IF, IP, ELISA and Antibody Microarray.

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Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-HSP90 beta Antibody [H9010]

Description

Mouse monoclonal [H9010] antibody to HSP90 beta.

Specificity

This antibody detects HSP90 beta specifically in all reactive species - except in Chicken, where it detects both alpha and beta isoforms.

Applications

WB, IHC, ICC/IF, IP, ELISA, Antibody Microarray

Dilutions

WB: 1:2500, IHC: 1:100

Reactivity

Human, Mouse, Rat, Rabbit, Chicken, Canine, Fish, Shark, Hamster

Immunogen

Recombinant human HSP90 beta protein.

Host

Mouse

Clonality

Monoclonal

Clone ID

H9010

Isotype

IgG2a

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

90 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.2, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

90 kda heat shock protein beta HSP90 beta, D6S182, FLJ26984, Heat shock 84 kDa, Heat shock 90kD protein 1, beta, Heat shock 90kDa protein 1 beta, Heat shock protein 90 alpha family class B member 1, Heat shock protein 90 kDa, Heat shock protein 90kDa alpha (cytosolic) class B member 1, Heat shock protein 90kDa alpha family class B member 1, Heat shock protein beta, Heat shock protein HSP 90 beta, Heat shock protein HSP 90-beta, HS90B_HUMAN, HSP 84, HSP 90, HSP 90 b, HSP 90b, HSP84, hsp90ab1, HSP90B, HSPC2, HSPCB

Isotype Controls

Suitable Secondaries

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (8)

Figure 1: Western Blot - Anti-HSP90 beta Antibody [H9010] (A305130)

Western blot analysis of human Lysates showing detection of Hsp90 protein using Anti-HSP90 beta Antibody [H9010] (A305130) at 1:1,000. Comparison of clone H9010 behavior with Hsp90 human beta (1) and Hsp90 human alpha (2).

Figure 2: Western Blot - Anti-HSP90 beta Antibody [H9010] (A305130)

Western blot analysis of human Cervical cancer cell line (HeLa) lysate showing detection of Hsp90 protein using Anti-HSP90 beta Antibody [H9010] (A305130) at 1:1,000. The secondary antibody used was HRP Goat Anti-Mouse.

Figure 3: Immunohistochemistry - Anti-HSP90 beta Antibody [H9010] (A305130)

Immunohistochemistry analysis of mouse backskin, fixed in Bouin's fixative solution and paraffin-embedded. The Primary Antibody used was Anti-HSP90 beta Antibody [H9010] (A305130) at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature. Localization: Epidermis.

Figure 4: Immunohistochemistry - Anti-HSP90 beta Antibody [H9010] (A305130)

Immunohistochemistry analysis of mouse inflamed colon, fixed in formalin. The Primary Antibody used was Anti-HSP90 beta Antibody [H9010] (A305130) at 1:10,000 for 12 hours at 4°C. The secondary antibody used was Biotin Goat Anti-Mouse at 1:2000 for 1 hour at room temperature. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at room temperature. Localization: Inflammatory cells. Magnification: 40x. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..

Figure 5: Immunohistochemistry - Anti-HSP90 beta Antibody [H9010] (A305130)

Immunohistochemistry analysis of human colon carcinoma, fixed in formalin. The Primary Antibody used was Anti-HSP90 beta Antibody [H9010] (A305130) at 1:10,000 for 12 hours at 4°C. The secondary antibody used was Alexa Fluor 555 Goat Anti-Mouse (red) at 1:5,000 for 1 hour at room temperature. Magnification: 40x. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..

Figure 6: Western Blot - Anti-HSP90 beta Antibody [H9010] (A305130)

Western blot analysis of human cell lysates from various cell lines showing detection of Hsp90 protein using Anti-HSP90 beta Antibody [H9010] (A305130) at 1:1,000 for 2 hours at room temperature. Load: 15 µg. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Sheep Anti-Mouse IgG: HRP for 1 hour at room temperature.

Figure 7: Immunohistochemistry - Anti-HSP90 beta Antibody [H9010] (A305130)

Immunohistochemistry analysis of human colon carcinoma, fixed in formalin. The Primary Antibody used was Anti-HSP90 beta Antibody [H9010] (A305130) at 1:10,000 for 12 hours at 4°C. The secondary antibody used was Biotin Goat Anti-Mouse at 1:2000 for 1 hour at room temperature. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at room temperature. Localization: Inflammatory cells. Magnification: 40x. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..

Figure 8: Immunohistochemistry - Anti-HSP90 beta Antibody [H9010] (A305130)

Immunohistochemistry analysis of mouse inflamed colon, fixed in formalin. The Primary Antibody used was Anti-HSP90 beta Antibody [H9010] (A305130) at 1:10,000 for 12 hours at 4°C. The secondary antibody used was Alexa Fluor 555 Goat Anti-Mouse (red) at 1:5,000 for 1 hour at room temperature. Localization: Inflammatory and epithelial mucosa. Magnification: 40x. Inflammatory and epithelial mucosa. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..

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