Recombinant Anti-Human IgG3 Antibody [RM119] (A121339)

Detection of human IgG3 in monkey serum, using Anti-Human IgG2 Antibody [RM119] (capture antibody) and Anti-Human Ig Light Chain Antibody [RM129] (Biotin) (detection antibody) as a Sandwich ELISA pair. HRP conjugated streptavidin and TMB were used to yield the colorimetric reaction.

Sandwich ELISA using Anti-Human IgG3 Antibody [RM119] as the capture antibody (100ng/well), and Anti-Human Ig Light Chain Antibody [RM129] (Biotin) as the detection antibody, followed by an HRP conjugated streptavidin.

ELISA showing Anti-Human IgG3 Antibody [RM119] reacts only to human IgG3, and not to any other IgG subclasses (IgG1, IgG2, or IgG4), and there is no cross reactivity to IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.

A titer ELISA using Anti-Human IgG3 Antibody [RM119]. The plate was coated with different amounts of human IgG3. A serial dilution of Anti-Human IgG3 Antibody [RM119] was used as the primary antibody, followed by an alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.