Human OPN ELISA Kit (A687)

Human OPN ELISA Kit

Sandwich (quantitative)

This OPN enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-OPN Antibody. Standards or samples are then added to the microtire plate wells and OPN, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of OPN present in the sample, Anti-OPN Antibody (Biotin) is added to each well to "sandwich" the OPN immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, Avidin-Biotin-Peroxidase Complex is added to each well for a short incubation period, followed by thorough washing of the wells to remove all unbound conjugates. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain OPN will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.

Microplate

Serum, plasma, body fluids, tissue lysates, and cell culture supernatants.

< 10 pg/ml

156-10,000 pg/ml

Human

Store at 2-8°C for 4 months or at -20°C for 8 months.

BNSP, Bone sialoprotein 1, BSP I, BSPI, Early T lymphocyte activation 1, ETA 1, ETA1, MGC110940, Nephropontin, Osteopontin, osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein, OSTP_HUMAN, PSEC0156, Secreted phosphoprotein 1, secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1), SPP 1, SPP-1, SPP1, SPP1/CALPHA1 fusion, Urinary stone protein, Uropontin