Human ST2 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human ST2 in the samples. A monoclonal antibody specific for human ST2 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for ST2, and ST2 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of ST2 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Platform

Microplate

Detection Type

Colorimetric

Sample Type

Cell culture supernatant, serum and plasma.

Sensitivity

1.10 pg/ml

Product Range

31.25 - 2000 pg/ml

Specificity

This kit can assay both natural and recombinant human ST2.

Reactivity

Human

Cross Reactivity

This assay has shown no significant cross-reactivity.

Storage

Store at 2 - 8°C.

Synonyms

DER-4, DER4, FIT 1, Growth stimulation expressed, homolog of mouse growth stimulation-expressed, Il1rl1, IL33R, ILRL1_HUMAN, Interleukin 1 receptor like 1, interleukin 1 receptor related protein, Interleukin-1 receptor-like 1, Protein ST2, ST2L, ST2V, T1, T1 protein

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.