Anti-Huntingtin Antibody (A308268)

Name: Anti-Huntingtin Antibody
Brand: Antibodies.com
SKU: A308268
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Huntingtin for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Huntingtin Antibody

Description

Rabbit polyclonal antibody to Huntingtin.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 435-635 of human Huntingtin (NP_002102.4).

Sequence

PVLSRKQKGKVLLGEEEALEDDSESRSDVSSSALTASVKDEISGELAASSGVSTPGSAGHDIITEQPRSQHTLQADSVDLASCDLTSSATDGDEEDILSHSSSQVSAVPSDPAMDLNDGTQASSPISDSSQTTTEGPDSAVTPSDSSEIVLDGTDNQYLGLQIGQPQDEDEEATGILPDEASEAFRNSSMALQQAHLLKNM

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

347 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AI256365, C430023I11Rik, HD, HD protein, HDH, HD_HUMAN, HTT, HUNTINGTON CHOREA, Huntington disease protein, Huntington's disease protein homolog, IT 15, IT15, OTTMUSP00000026909, ZHD

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-Huntingtin Antibody (A308268)

Western blot analysis of extracts of various cell lines, using Anti-Huntingtin Antibody (A308268) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Immunohistochemistry - Anti-Huntingtin Antibody (A308268)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-Huntingtin Antibody (A308268) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunofluorescence - Anti-Huntingtin Antibody (A308268)

Immunofluorescence analysis of U-251 MG cells using Anti-Huntingtin Antibody (A308268) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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