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Anti-ICAM1 Antibody (A9725)

Name
Anti-ICAM1 Antibody
Description
Rabbit polyclonal antibody to ICAM1.
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:1,000-1:5,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 120-320 of human ICAM-1/CD54 (NP_000192.2).
Sequence
PLPSWQPVGKNLTLRCQVEGGAPRANLTVVLLRGEKELKREPAVGEPAEVTTTVLVRRDHHGANFSCRTELDLRPQGLELFENTSAPYQLQTFVLPATPPQLVSPRVLEVDTQGTVVCSLDGLFPVSEAQVHLALGDQRLNPTVTYGNDSFSAKASVSVTAEDEGTQRLTCAVILGNQSQETLQTVTIYSFPAPNVILTKP
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
89 kDa / 92 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Antigen identified by monoclonal, BB 2, BB2, CD 54 , CD54, CD54 antigen , CD_antigen=CD54, Cell surface glycoprotein P3.58 , Human rhinovirus receptor , ICAM 1, ICAM-1, ICAM1_HUMAN, Intercellular adhesion molecule 1 , intercellular adhesion molecule 1 (CD54), human rhinovirus receptor, Major group rhinovirus receptor , MALA 2, MALA2 , MGC6195, MyD 10, MyD10, P3.58 , Surface antigen of activated B cells , Surface antigen of activated B cells, BB2
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-ICAM1 Antibody (A9725) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-ICAM1 Antibody (A9725)
Western blot analysis of various lysates, using Anti-ICAM1 Antibody (A9725) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 0.5s.
Western Blot - Anti-ICAM1 Antibody (A9725) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-ICAM1 Antibody (A9725)
Western blot analysis of various lysates, using Anti-ICAM1 Antibody (A9725) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 0.5s.
Western Blot - Anti-ICAM1 Antibody (A9725) - Antibodies.comEnlarge Image
Figure 3: Western Blot - Anti-ICAM1 Antibody (A9725)
Western blot analysis of Mouse lung, using Anti-ICAM1 Antibody (A9725) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 0.5s.
Immunohistochemistry - Anti-ICAM1 Antibody (A9725) - Antibodies.comEnlarge Image
Figure 4: Immunohistochemistry - Anti-ICAM1 Antibody (A9725)
Immunohistochemistry analysis of paraffin-embedded mouse lung using Anti-ICAM1 Antibody (A9725) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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