Anti-ID1 Antibody [PCRP-ID1-2F11] (A277652)

Mouse monoclonal [PCRP-ID1-2F11] antibody to ID1 for ELISA, IP, Flow Cytometry and IF.

Alternative Formats and ConjugatesAlternative Formats

Freight Charges
Lead Time
4-6 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Anti-ID1 Antibody [PCRP-ID1-2F11]
Description
Mouse monoclonal [PCRP-ID1-2F11] antibody to ID1.
Specificity
Members of the Id family of basic helix-loop-helix (bHLH) proteins include Id1, Id2, Id3 and Id4. They are ubiquitously expressed and dimerize with members of the class A and B HLH proteins. Due to the absence of the basic region, the resulting heterodimers cannot bind DNA. The Id-type proteins thus appear to negatively regulate DNA binding of bHLH proteins. Since Id1 inhibits DNA binding of E12 and Myo D, it apparently functions to inhibit muscle-specific gene expression. Under conditions that facilitate muscle cell differentiation, the Id protein levels fall, allowing E12 and/or E47 to form heterodimers with Myo D and myogenin, which in turn activate myogenic differentiation. It has been shown that expression of each of the Id proteins is strongly dependent on growth factor activation and that reduction of Id mRNA levels by antisense oligonucleotides leads to a delayed reentry of arrested cells into the cell cycle following growth factor stimulation.
Applications
ELISA, IP, Flow Cytometry, IF
Dilutions
IP: 1-2µg / 100-500µg proteins, Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml
Reactivity
Human
Immunogen
Recombinant fragment, around amino acids 5-150, of human ID1 protein. The exact sequence is proprietary.
Host
Mouse
Clonality
Monoclonal
Clone ID
PCRP-ID1-2F11
Isotype
IgG1
Conjugate

Unconjugated

Purification
Protein A/G chromatography.
Concentration
200 µg/ml
Molecular Weight
16.13 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
General Notes
This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-ID1 Antibody [PCRP-ID1-2F11] - BSA and Azide free (A278240).
Synonyms
bHLHb24, Class B basic helix-loop-helix protein 24, dJ857M17.1.2 (inhibitor of DNA binding 1, dominant negative helix-loop-helix protein), DNA binding protein inhibitor ID 1, DNA binding protein inhibitor ID1, DNA-binding protein inhibitor ID-1, Dominant negative helix loop helix protein, ID, ID 1, ID1_HUMAN, Inhibitor of Differentiation 1, Inhibitor of DNA binding 1, inhibitor of DNA binding 1, dominant negative helix-loop-helix protein
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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