Figure 1: Western Blot - Anti-IgE Antibody [ARC60081] (A309727)
Western blot analysis of various lysates, using Anti-IgE Antibody [ARC60081] (A309727) at 1:2,000 dilution. The secondary antibody was HRP Goat Anti-Mouse IgG (H+L) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 120s.
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