ED50: 5-25 ng/ml, as determined by the dose-dependent proliferation of human MCF/7 cells , in the cell culture mixture dependent on culture conditions. Its activity consists of 30-40 % compared to human IGF-1.
Purity
>98%.
Product Form
Lyophilized
Concentration
0.5mg/ml
Formulation
Lyophilized from a solution containing 0.02% Sodium Bicarbonate.
Reconstitution
Reconstitute with 0.4% Sodium Bicarbonate adjusted to pH 8-9, to no less than 0.1 mg/ml, which can then be further diluted to other aqueous solutions.
Storage
Shipped at ambient temperature. Lyophilized: Short term (3 weeks) store at 25°C. Long term store desiccated below -18°C. Reconstituted: Short term (2-7 days) store at 4°C. Long term store below -18°C. Avoid freeze/thaw cycles.